Carboxymethylation of ulvan and chitosan and their use as polymeric components of bone cements

last updated: 2013-10-23
TitleCarboxymethylation of ulvan and chitosan and their use as polymeric components of bone cements
Publication TypePapers in Scientific Journals
Year of Publication2013
AuthorsBarros, A. A., Alves A., Nunes C., Coimbra M. A., Pires R. A., and Reis R. L.

We carboxymethylated ulvan, extracted from the green algae Ulva lactuca, and chitosan, extracted fromLoligo forbesis squid-pen, yielding polysaccharides with an average degree of substitution of ∼98% (carboxymethyl ulvan, CMU) and ∼87% (carboxymethyl chitosan, N,O-CMC). The carboxymethylation was confirmed by FTIR and quantified by conductimetric titration and 1H NMR. The average molecular weight increased with the carboxymethylation (chitosan: Mn 145→296 kDa and Mw 227→416 kDa; ulvan: Mn139→261 kDa and Mw 368→640 kDa) indicating successful chemical modifications. Mixtures of the modified polysaccharides were tested in the formulation of polyacrylic acid-free glass-ionomer bone cements. Mechanical and in vitro bioactivity tests indicate that the inclusion of CMU in the cement formulation, i.e. 0.50:0.50 N,O-CMC:CMU, enhances its mechanical performance (compressive strength of 52.4 ± 8.0 MPa and modulus of 2.3 ± 0.3 GPa), generates non-cytotoxic cements and induces the diffusion of Ca and/or P-based moieties from the surface to the bulk of the cements.

JournalActa Biomaterialia
Date Published2013-06-21
KeywordsBone cements, ionomer cements
Peer reviewedyes

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