The culture of cells in a three-dimensional (3D) scaffold has been reported as a key issue influencing the newly formed tissue organization, either in vitro or in vivo. Scaffolds can present different material chemistry, geometry, structure, physical properties and degradation, as it has been explored in several chapters of this book.
Herein, we intend to give the reader an overview of the most used tests for evaluation of cells cytotoxicity and their response to natural materials in vitro.