Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Tuning Enzymatically-crosslinked Silk fibroin Hydrogel Properties for the Development of a Colorectal cancer Extravasation 3D Model on a Chip

Abstract

Microfluidic devices are now the most promising tool to mimic in vivo like scenarios such as tumorigenesis and metastasis due to its ability to more closely mimic cell’s natural microenvironment (such as three dimensional (3D) environment and continuous perfusion of nutrients). In this study, the ability of 2% and 3% enzymatically-crosslinked silk fibroin (eSF) hydrogels with different mechanical properties were tested in terms of colorectal cancer cell migration, under different microenvironments in a 3D dynamic model.  Matrigel was used as control. Moreover, we present a comprehensive comparison between the traditional Boyden chamber assay and our 3D dynamic microfluidic model in terms of colorectal cancer cell migration. Our results show profound differences between the two used biomaterials and the two migration models, which were explored in terms of mechanical properties of the hydrogels as well as the intrinsic characteristics of the models. Moreover, we validated the developed 3D dynamic model by demonstrating that hVCAM-1 plays a major role in the extravasation process, influencing extravasation rate and traveled distance. Furthermore, the developed model enables precise visualization of cancer cell migration within a 3D matrix in response to microenvironmental cues, shedding light on the importance of biophysical properties in cell behavior.

Journal
Global Challenges
Volume
2
Issue
5-6
Pagination
1700100
Publisher
Wiley
ISSN
2056-6646
URL
https://onlinelibrary.wiley.com/doi/epdf/10.1002/gch2.201700100
Keywords
3D model, Colorectal cancer, extravasion, Microfluidics, silk
Rights
Open Access
Peer Reviewed
Yes
Status
published
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