A soft 3D polyacrylate hydrogel recapitulates the cartilage niche and allows growth-factor free tissue engineering of human articular cartilage

last updated: 2019-04-23
ProjectN/A :: publications list
TitleA soft 3D polyacrylate hydrogel recapitulates the cartilage niche and allows growth-factor free tissue engineering of human articular cartilage
Publication TypePapers in Scientific Journals
Year of Publication2019
AuthorsJiménez G., Venkateswaran S., López-Ruiz E., Perán M., Pernagallo S., Díaz-Monchón J. J., Canadas R. F., Antich C., Dr. Oliveira J. M., Callanan A., Walllace R., Reis R. L., Montañez E., Carrillo E., Bradley M., and Marchal J. A.
EditorsWagner W. R.
Abstract

Cartilage degeneration or damage treatment is still a challenge, but, tissue engineering strategies, which combine cell therapy strategies, which combine cell therapy and scaffolds, and have emerged as a promising new approach. In this regard, polyurethanes and polyacrylates polymers have been shown to have clinical potential to treat osteochondral injuries. Here, we have used polymer microarraystechnology to screen 380 different polyurethanes and polyacrylates polymers. The top polymers with potential to maintain chondrocyte viability were selected, with scale-up studies performed to evaluate their ability to support chondrocyte proliferation during long-term culture, while maintaining their characteristic phenotype. Among the selected polymers, poly (methylmethacrylate-co-methacrylic acid), showed the highest level of chondrogenic potential and was used to create a 3D hydrogel. Ultrastructural morphology, microstructure and mechanical testing of this novel hydrogel revealed robust characteristics to support chondrocyte growth. Furthermore, in vitro and in vivo biological assays demonstrated that chondrocytes cultured on the hydrogel had the capacity to produce extracellular matrix similar to hyaline cartilage, as shown by increased expression of collagen type IIaggrecan and Sox9, and the reduced expression of the fibrotic marker’s collagen type I. In conclusion, hydrogels generated from poly (methylmethacrylate-co-methacrylic acid) created the appropriate niche for chondrocyte growth and phenotype maintenance and might be an optimal candidate for cartilage tissue-engineering applications.

JournalActa Biomaterialia
Volume19
Edition1
Issue30215
Pagination1-11
Date Published2019-03-22
PublisherElsevier
ISSN1742-7061
DOI10.1016/j.actbio.2019.03.040
URLhttps://www.sciencedirect.com/science/article/pii/S1742706119302156
Keywordscartilage tissue engineering, hydrogel, Poly (methylmethacrylate-co-methacrylic acid), Polyacrylate, Polymer microarray
RightsembargoedAccess (2 Years)
Peer reviewedyes
Statuspublished

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