Modulation of inflammation by anti-TNF α mAb-dendrimer nanoparticles loaded in tyramine-modified gellan gum hydrogels in a cartilage-on-a-chip model

last updated: 2021-05-17
ProjectTERM - Programa de Doutoramento FCT :: publications list
TitleModulation of inflammation by anti-TNF α mAb-dendrimer nanoparticles loaded in tyramine-modified gellan gum hydrogels in a cartilage-on-a-chip model
Publication TypePapers in Scientific Journals
Year of Publication2021
AuthorsOliveira I. M., Carvalho M. R., Fernandes D. C., Abreu C. M., Maia F. R., Pereira H., Caballero D., Kundu S. C., Reis R. L., and Dr. Oliveira J. M.
Abstract

Rheumatoid arthritis (RA) is an autoimmune and chronic inflammatory disease characterized by joint inflammation. Since the inflammatory condition plays an important role in the disease process, it is important to develop and test new therapeutic approaches that specifically target and treat joint inflammation. In this study, a human 3D inflammatory cartilage-on-a-chip model was established to test the therapeutic efficacy of anti-TNFα mAb-CS/PAMAM dendrimer NPs loaded-Tyramine-Gellan Gum in the treatment of inflammation. The results showed that the proposed therapeutic approach applied to the human monocyte cell line (THP-1) and human chondrogenic primary cells (hCH) cell-based inflammation system revealed an anti-inflammatory capacity that increased over 14 days. It was also possible to observe that Coll type II was highly expressed by inflamed hCH upon the culture with anti-TNF α mAb-CS/PAMAM dendrimer NPs, indicating that the hCH cells were able maintain their biological function. The developed preclinical model allowed us to provide more robust data on the potential therapeutic effect of anti-TNF α mAb-CS/PAMAM dendrimer NPs loaded-Ty-GG hydrogel in a physiologically relevant model.

JournalJournal Of Materials Chemistry B
Date Published2021-05-10
ISSN2050-7518
DOI10.1039/D1TB00802A
Keywordsdrug screening, Inflammatory Cartilage Model, Microfluidics, Ty-GG Hydrogel; Dendrimer nanoparticles
RightsopenAccess
Peer reviewedno
Statuspublished

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