Background: This study investigated the preparation of ordered patterned surfaces and/or microspheres from a natural-based polymer, using the breath figure and reverse breath figure methods.
Methods: Poly(D,L-lactic acid) and starch poly(lactic acid) solutions were precipitated in different conditions – namely, polymer concentration, vapor atmosphere temperature and substrate – to evaluate the effect of these conditions on the morphology of the precipitates obtained.
Results: The possibility of fine-tuning the properties of the final patterns simply by changing the vapor atmo- sphere was also demonstrated here using a range of compositions of the vapor phase. Porous films or discrete particles are formed when the differences in surface tension determine the ability of polymer solution to sur- round water droplets or methanol to surround polymer droplets, respectively. In vitro cytotoxicity was assessed applying a simple standard protocol to evaluate the possibility to use these materials in biomedical applications. Moreover, fluorescent microscopy images showed a good interaction of cells with the material, which were able to adhere on the patterned surfaces after 24 hours in culture.
Conclusions: The development of patterned surfaces using the breath figure method was tested in this work for the preparation of both poly(lactic acid) and a blend containing starch and poly(lactic acid). The potential of these films to be used in the biomedical area was confirmed by a preliminary cytotoxicity test and by morphological observation of cell adhesion.