Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

The effects of platelet lysate patches on the activity of tendon-derived cells

Abstract

Platelet-derived biomaterials are widely explored as cost-effective sources of therapeutic factors, holding a strong potential for endogenous regenerative medicine. Particularly for tendon repair, treatment approaches that shift the injury environment are explored to accelerate tendon regeneration. Herein, genipin-crosslinked platelet lysate (PL) patches are proposed for the delivery of human-derived therapeutic factors in patch augmentation strategies aiming at tendon repair. Developed PL patches exhibited a controlled release profile of PL proteins, including bFGF and PDGF-BB. Additionally, PL patches exhibited an antibacterial effect by preventing the adhesion, proliferation and biofilm formation by S. aureus, a common pathogen in orthopaedic surgical site infections. Furthermore, these patches supported the activity of human tendon-derived cells (hTDCs). Cells were able to proliferate over time and an up-regulation of tenogenic genes (SCXCOL1A1 and TNC) was observed, suggesting that PL patches may modify the behavior of hTDCs. Accordingly, hTDCs deposited tendon-related extracellular matrix proteins, namely collagen type I and tenascin C.

In summary, PL patches can act as a reservoir of biomolecules derived from PL and support the activity of native tendon cells, being proposed as bioinstructive patches for tendon regeneration.

Journal
Acta Biomaterialia
ISSN
1742-7061
URL
https://www.sciencedirect.com/science/article/pii/S1742706118300175
Keywords
Antibacterial efficacy, controlled release, Endogenous regeneration, tendon patch, Tenogenic genes
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
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