Biomaterials, Biodegradables and Biomimetics Research Group

Book Chapter

Decellularized hASCs-derived matrices as biomaterials for 3D in vitro approaches

Abstract

Tissue regeneration strategies have been greatly evolving in the last years due to the use of more realistic approaches. These approaches rely in the use of biomaterials for the development of three dimensional (3D) structures that emulate the in vivo microenvironment of different tissues. Recently, extracellular matrices (ECM) secreted by cells have been caught a great deal of attention as an attractive biomaterial for the development 3D structures. In fact, different cells and/or different cellular culture conditions gave rise to different ECM's compositions, which can be used for the development of more physiologically relevant 3D structures. Nevertheless, the recovery of cell-derived ECM requires the use of a proper decellularization protocol. Herein, we report a decellularization protocol to recover the ECM produced by human adipose derived stem cells. This protocol comprises multiple steps (chemical, physical or enzymatic) which are described here in more detail. Furthermore, it is describe the methods that have been used to evaluate the effectiveness of this decellularization protocol. Overall, this protocol enables the production of hASCs-derived matrices that can be further used for the production of more physiologically relevant 3D in vitro models for tissue regeneration strategies.

Journal
Methods in Cell Biology - Cell-derived Matrices Part B
Volume
157
Edition
1st
Pagination
3-21
Publisher
Elsevier
ISBN
0091-679X
URL
http://www.sciencedirect.com/science/article/pii/S0091679X19301499
Keywords
Cell-derived matrices, Decellularization, Extracellular matrix, Natural biomaterials, regenerative medicine
Rights
Closed Access
Peer Reviewed
Yes
Status
published
This website uses cookies. By using this website you consent to our use of these cookies. For more information visit our Policy Page.