Biomaterials, Biodegradables and Biomimetics Research Group

Comunication - Oral

Chondrogenesis-inductive nanofibrous substrate comprising autologous TGF-b3 and IGF-I from platelet lysates

Abstract

Articular cartilage is a connective tissue with low self-regeneration potential due to its avascular nature and lack of progenitor cells [1]. Therefore, cartilage regeneration is far to be achieved and then it is an important candidate for tissue engineering and regenerative medicine (TERM) strategies. TGF-b3 and IGF-I have been identified as important proteins on the regulation of cartilage development and on the homeostasis of mature articular cartilage [2, 3]. Therefore, the synergetic effect of the growth factors (GFs) in a biomaterial substrate is hypothesized to lead to the successful chondrogenic differentiation of mesenchymal stem cells (MSCs). The leading goal of this study is to develop a multi-functionalized electrospun nanofibrous mesh (NFM) with chondrogenic potential, through the binding of autologous TGF-b3 and IGF-I. For that, defined antibodies were immobilized at the surface of NFMs, capable to specifically select only the GFs of interest from a complex biological fluid (i.e. platelet lysate - PL). The maximum immobilization capacity of each antibody was 4 mg/mL for both antibodies. The anti-TGF-b3 and anti-IGF-I antibodies were also immobilized over the same structure in a mixed fashion at 1:10 proportion (0.4mg/mL anti-TGF-b3 : 3.6mg/mL anti-IGF-I). The bioactivity of immobilized antibodies was tested by using recombinant proteins (rGF), achieving a binding efficiency of 36% for TGF-b3 and 44% for IGF-I. Similar results were observed for GFs captured from PL by NFMs functionalized with single or mixed antibodies. The chondrogenic potential of this bi-functionalized biomaterial system (Single or Mixed, with TGF-b3 and IGF-I captured from PL or rGF) was further assessed by culturing human bone marrow-derived MSCs during 28 days without further induction. Bare NFMs cultured with hBMSCs under standard chondrogenic differentiation medium supplemented with TGF-b3 and IGF-I were used as positive control. Biological results indicate that the bio-functional systems are more effective when compared to control condition.

[1] Correa, D. and S.A. Lietman, Semin Cell Dev Biol, 2016; [2] Petrou, M., et al., Regen Med, 2013; [3] Longobardi, L., et al., J Bone Miner Res, 2006.

Journal
CHEM2NATURE First School
Keywords
chondrogenic differentiation, growth factors, Nanofibrous meshes, Platelet lysate
Rights
Open Access
Peer Reviewed
Yes
Status
published
Project
PATH
Year of Publication
2016
Date Published
2016-11-21
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