Scientists can now envision a wide range of possibilites when addressing tissue and organ regeneration due to the great developments in the biomaterials field. At the 3B's Research Group, we have been developing several natural-based biomaterials (e.g. chitosan, gellan gum, silk fibroin, alginates, and kefiran) with the required functionalities for further processing as micro- and nano-particles, hydrogels, membranes, capsules, scaffolds, and bioinks. Herein, the most relevant advances of biomaterials functionalization and processing methods for further application in musculoskeletal, neurosciences and cancer research will be presented.