Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Surface microstructuring and protein patterning using hyaluronan derivatives

Abstract

Natural polymers, such as hyaluronan, are considered as good candidates to substitute synthetic materials in many biological applications due to their intrinsic biocompatibility for in vitro and living cell experiments. This work describes surface modifications performed over modified hyaluronan derivatives which could help to understand the physical and biochemical cues on the cell-behaviour. A photocrosslinkable methacrylated hyaluronan was microstructured using soft lithography techniques to obtain a microenvironment suitable for cell-behaviour experiments, which might mimic the extra-cellular matrix. In addition, sulphated and non-sulphated oxidized hyaluronan were immobilized on bare substrates and micrometric features of cell adhesive and non-adhesive proteins were patterned by microcontact printing on top of them. The obtained structures were characterized by optical microscopy, profilometry and atomic force microscopy. The stability of structures was tested by immersion in physiological salt solutions. The observed results prove the suitability of the materials and protocols described.

Journal
Microelectronic Engineering
Volume
106
Pagination
21-26
Publisher
Elsevier
URL
http://www.sciencedirect.com/science/article/pii/S0167931713000671
Keywords
Glycosaminoglycan, Hyaluronan, hydrogel, Microcontact printing, Microstructuring, Photocrosslinking
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
Year of Publication
2013
DOI
10.1016/j.mee.2013.01.039
Date Published
2013-02-08
Search Google ScholarGenerate BibTexDownload RTF
This website uses cookies. By using this website you consent to our use of these cookies. For more information visit our Policy Page.