Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Silk hydrogels from non-mulberry and mulberry silkworm cocoons processed with ionic liquids

Abstract

Matrices based on silk fibroin from the non-mulberry silkworm Antheraea mylitta and the mulberry silkworm Bombyx mori have demonstrated good applicability in regenerative medicine. However, the cocoons of A. mylitta are underutilized in part due to their lack of solubility in traditional organic solvents. Therefore, the present work investigates the solubilization and processing of degummed fibers obtained from the cocoons of both silkworm species into hydrogels using ionic liquids (ILs). The developed hydrogels exhibited a rubbery consistency, viscoelastic behavior and rapid degradation in the presence of protease XIV. Scanning electron and confocal microscopy images suggest that human adipose stem cells (hASCs) are able to adhere to and migrate at different levels within the hydrogel structures. Moreover, the MTS assay demonstrated the maintenance of cell metabolic activity for up to 28days, while DNA quantification showed that hASCs were able to proliferate on the seeded hydrogels. The findings indicate that complete IL removal from the fabricated hydrogels results in a positive hASCs cellular response. Thus the present approach provides a unique opportunity to broaden the processability and application of silk fibroin obtained from A. mylitta cocoons for regenerative medicine, namely cartilage regeneration.

Journal
Acta Biomaterialia
Volume
9
Issue
11
Pagination
8972-8982
Publisher
Elsevier
ISSN
1742-7061
URL
http://www.sciencedirect.com/science/article/pii/S1742706113003371
Keywords
3D scaffolds, ionic liquids, Silk Fibroin
Rights
Open Access
Peer Reviewed
Yes
Status
published
This website uses cookies. By using this website you consent to our use of these cookies. For more information visit our Policy Page.