In this work, the authors propose to explore the binding potential of nonostructured multilayers produced by layer-bylayer
self-assembly based on perfusion technique, which was otherwise limited to coating only. Random spherical wax
templates of same size were transformed into moldable soft three-dimensional (3D) core material with different external
geometries. Placement of template spheres with different sizes by sequential stacking along multiple space axes in a
single construct induced internal variations leading to different patterns. Stable porous interconnected nanostructured
soft 3D macroconstructs made of natural based polyelectrolytes with a controlled external shape and different internal
patterns were developed after removing wax template. Such highly porous structures with hierarchical organization
could be used, for example, as scaffolds for tissue engineering applications