Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Manipulation of chemical composition and architecture of non-biodegradable poly(ethylene terephthalate)/chitosan fibrous scaffolds and their effects on L929 cell behavior

Abstract

Microporous, non-woven fibrous scaffolds made of poly(ethylene terephthalate) and chitosan were produced by electrospinning. Fiber morphology, diameter, pore size, and wettability were manipulated by varying the chemical composition of the electrospinning solution, i.e. chitosan concentration and molecular weight, and by post-electrospinning treatment with glutaraldehyde. In vitro studies were conducted using a fibroblast cell line toward a comprehensive understanding of how scaffolds characteristics can modulate the cell behavior, i.e. viability, adhesion, proliferation, extracellular matrix secretion, and three-dimensional colonization. Substantial differences were found as a result of scaffold morphological changes. Higher levels of adhesion, spreading, and superficial proliferation were achieved for scaffolds with smaller fiber and pore diameters while cell penetration and internal colonization were enhanced for scaffolds with larger pores. Additionally, the available area for cell adhesion, which is related to fiber and pore size, was a crucial factor for the viability of L929 cells. This paper provides significant insights for the development and optimization of electrospun scaffolds toward an improved biological performance.

Journal
Materials Science and Engineering C
Volume
33
Pagination
37–46
Keywords
adhesion, Chitosan, Electrospinning, Poly(ethylene terephthalate), Proliferation, Scaffold.
Rights
Open Access
Peer Reviewed
Yes
Status
published
Year of Publication
2012
Date Published
2012-08-11
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