In recent years, progress in the field of hybrid materials has been accelerated through use of
the sol–gel process for creating materials and devices, which benefit from the incorporation of
both inorganic and organic components. In this work, organic–inorganic hybrid membranes were
prepared from tetraethoxysilane and a blend system composed of chitosan and soy protein. By
introducing a small amount of siloxane bond into the chitosan/soy protein system, the chitosan/
soy protein hybrid membranes were improved in terms of structure, topography and mechanical
properties. It appears that the chitosan/soy protein hybrid membranes were formed by discrete
inorganic moieties entrapped in the chitosan/soy protein blend, which improved the stability and
mechanical performance assessed by the dynamic mechanical analysis as compared to chitosan/
soy protein membrane. Also, in vitro cell culture studies evidenced that the chitosan/soy protein
hybrid membranes are non-cytotoxic over a mouse fibroblast-like cell line. The hybrid membranes
of silane-treated chitosan/soy protein developed in this work have potential in biomedical
applications, including tissue engineering.