The success of many stem cell applications in the biomedical field is highly dependent from efficient separation techniques for isolation and purification of cell populations with a very high yield and purity. Effective strategies to select subsets of cells, decreasing cell manipulation and expansion time to obtain large quantities of a particular cell type are urgently needed. Microcarrier culture system offers the advantage of providing a larger surface area for the growth of anchorage-dependent cells. Several microcarrirers have been used for cell expansion however none of the existing systems is able to select specific cell types from a heterogeneous population. Our group developed chitosan films grafted with antibodies for selective cell attachment. Herein we present chitosan microparticles (μPs) able to capture a specific cell types based in the concept of antibody coating. Such system acts also as a carrier for cell expansion of a specific cell population and may be used to directly deliver cells in vivo to repair and regenerate tissues.