Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Dynamic Culture of Osteogenic Cells in Biomimetically Coated Poly(Caprolactone) Nanofibre Mesh Constructs

Abstract

n our previous work, biomimetic calcium phosphate–coated poly(caprolactone) nanofibre meshes (BCP-NMs) were demonstrated to be more effective for supporting cell attachment and proliferation under static conditions, when compared with poly(caprolactone) nanofibre meshes (PCL-NMs). In many applications, in vitro cultivation of constructs using bioreactors that support efficient nutrition of cells has appeared as an important step toward the development of functional grafts. This work aimed at studying the effects of dynamic culture conditions and biomimetic coating on bone cells grown on the nanofibre meshes. BCP-NM and PCL-NM were seeded with osteoblast-like cells (MG63—human osteosarcoma-derived cell line). The cell-seeded constructs were cultured within a rotating bioreactor that simulated microgravity, at a fixed rotating speed, for different time periods, and then characterized. Cell morphology, viability, and phenotype were assessed. PCL-NM constructs presented a higher number of dead cells than BCP-NM constructs. Under dynamic conditions, the production of proteins associated with the extracellular matrix of bone was higher on BCP-NM constructs than in the PCL-NM ones, which indicates that coated samples may provide cells with a better environment for tissue growth. It is suggested that improved mass transfer in the bioreactor in combination with the appropriate substrate were decisive factors for this highly positive outcome for generating bone.

Journal
TISSUE ENGINEERING: Part A
Volume
16
Issue
2
Pagination
557-563
Keywords
Dynamic culture, nanofibres, osteogenic
Rights
Open Access
Peer Reviewed
Yes
Status
published
Year of Publication
2010
Date Published
2010-10-13
Search Google ScholarGenerate BibTexDownload RTF
This website uses cookies. By using this website you consent to our use of these cookies. For more information visit our Policy Page.