Carboxymethyl-chitosan/Poly(amidoamine) Dendrimer Nanoparticles in Central Nervous System Regenerative Medicine: Effects on Neurons/Glial Cells Viability and Internalization efficiency

last updated: 2014-12-10
TitleCarboxymethyl-chitosan/Poly(amidoamine) Dendrimer Nanoparticles in Central Nervous System Regenerative Medicine: Effects on Neurons/Glial Cells Viability and Internalization efficiency
Publication TypePapers in Scientific Journals
Year of Publication2011
AuthorsSalgado A. J., Dr. Oliveira J. M., Pirraco R. P., Pereira V. H., Fraga J. S., Marques A. P., Neves N. M., Mano J. F., Reis R. L., and Sousa N.
Abstract

The applicability of CMCht/PAMAM dendrimer nanoparticles for CNS applications was investigated. AFM and TEM observations revealed that the nanoparticles possessed a nano- sphere-like shape with a size from 22.0 to 30.7nm. The nanoparticles could be bound to fluorescent-probe FITC for tracing purposes. Post-natal hippocampal neurons and cortical glial cells were both able to internalize the FITC-labeled

CMCht/PAMAM dendrimer nanoparticles with high efficiency. The percentage of positive cells internalizing the nanoparticles varied, reaching a peak after 48h of incubation. Further exper- iments for periods up to 7d revealed that the periodical addition of FITC-labelled CMCht/ PAMAM dendrimer nanoparticles was needed to maintain the overall percentage of cells interna- lizing them. Finally, it was also observed that cell viability was not significantly affected by the incubation of dendrimer nanoparticles.

JournalMacromolecular Bioscience
Volume10
Issue10
Pagination1130-1140
Date Published2011-02-15
KeywordsCentral nervous system, Dendrimers
RightsopenAccess
Peer reviewedyes
Statuspublished

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