@article {19850,
	title = {Colorectal tumor-on-a-chip system: A 3D tool for precision onco-nanomedicine},
	journal = {Science Advances},
	volume = {5},
	year = {2019},
	month = {2019-05-22 00:00:00},
	publisher = {American Association for the Advancement of Science.},
	abstract = {
Awareness that traditional two-dimensional (2D) in vitro and nonrepresentative animal models may not completely
emulate the 3D hierarchical complexity of tissues and organs is on the rise. Therefore, posterior
translation into successful clinical application is compromised. To address this dearth, on-chip biomimetic microenvironments\ powered by microfluidic technologies are being developed to better capture the complexity\ of in vivo pathophysiology. Here, we describe a {\textquotedblleft}tumor-on-a-chip{\textquotedblright} model for assessment of precision nanomedicine\ delivery on which we validate the efficacy of drug-loaded nanoparticles in a gradient fashion. The model\ validation was performed by viability studies integrated with live imaging to confirm the dose-response effect\ of cells exposed to the CMCht/PAMAM nanoparticle gradient. This platform also enables the analysis at the gene\ expression level, where a down-regulation of all the studied genes (MMP-1, Caspase-3, and Ki-67) was observed.
This tumor-on-chip model represents an important development in the use of precision nanomedicine toward
personalized treatment.
},
	keywords = {3D, Cancer, Microfluidics, Nanoparticles},
	issn = {2375-2548},
	doi = {10.1126/sciadv.aaw1317},
	url = {https://advances.sciencemag.org/content/5/5/eaaw1317.full},
	author = {Carvalho, M. R. and Barata, D. and Teixeira, L. M. and Giselbrecht, S. and Reis, R. L. and Oliveira, J. M. and Truckenm{\"u}ller, R. and Habibovic, P.}
}