@article {19727,
	title = {Chondrogenesis-inductive nanofibrous substrate using both biological fluids and mesenchymal stem cells from an autologous source},
	journal = {Materials Science and Engineering: C},
	volume = {98},
	year = {2019},
	month = {2019-01-16 00:00:00},
	pages = {1169-1178},
	publisher = {ELSEVIER},
	abstract = {
During the last decade, many cartilage tissue engineering strategies have been developed, being the stem cell-based approach one of the most promising. Transforming Growth Factor-β3 (TGF-β3) and Insulin-like Growth Factor-I (IGF-I) are key proteins involved in the regulation of chondrogenic differentiation. Therefore, these two growth factors (GFs) were immobilized at the surface of a single electrospun nanofibrous mesh (NFM) aiming to differentiate human Bone Marrow-derived Mesenchymal Stem Cells (hBM-MSCs). The immobilization of defined antibodies (i.e. anti-TGF-β3 and anti-IGF-I) allows the selective retrieval of the abovementioned GFs from human platelet lysates (PL). Biochemical assays, involving hBM-MSCs cultured on biofunctional nanofibrous substrates under basal culture medium during 28 days, confirm the biological activity of bound TGF-β3 and IGF-I. Specifically, the typical spherical morphology of chondrocytes and the immunolocalization of collagen type II confirmed the formation of a cartilaginous ECM. Therefore, the proposed biofunctional nanofibrous substrate is able to promote chondrogenesis.
},
	keywords = {chondrogenic differentiation, Electrospun nanofibrous meshes, Insulin-like growth factor-I (IGF-I), platelet lysates, Transforming growth factor-β3 (TGF-β3)},
	issn = {0928-4931},
	doi = {10.1016/j.msec.2019.01.069},
	url = {https://doi.org/10.1016/j.msec.2019.01.069.},
	author = {Casanova, M. R. and Alves da Silva, M. and Costa-Pinto, A. R. and Reis, R. L. and Martins, A. and Neves, N. M.},
	editor = {Kim, D. - H.}
}