@inbook {18951,
	title = {Advanced in-vitro cell culture methods using natural biomaterials},
	booktitle = {Biomaterials from Nature for Advanced Devices and Therapies},
	volume = {1},
	year = {2016},
	month = {2016-10-01 00:00:00},
	pages = {532 - 561},
	publisher = {Wiley},
	organization = {Wiley},
	edition = {1},
	chapter = {29},
	abstract = {
Abstract
The in vitro culturing of constructs for Tissue Engineering is a crucial step towards it future application in vivo. Not only the cells response to the biomaterials must be studied, but also the way cells interact with the scaffold in a specific tissue development context. In this chapter, we will provide an overview of the most advanced in vitro cell culture methods using natural-based biomaterials. We will explore the use of bioreactors as tools to scale-up biomaterials and cell-scaffold constructs, and also their use in the mimicking of a cellular microenvironment. The culture conditions of cells and scaffolds is another important issue, as different tissues require different oxygen tensions, or alternatively, show improved ability to regenerate when co-cultured with other types of cells. The recent advances in gene and drug delivery, using scaffolds and nanoparticles, are also explored herein.
},
	keywords = {Advanced Therapies, in vitro studies},
	isbn = {978-1-118-47805-9},
	author = {Alves da Silva, ML and Reis, R. L. and Neves, N. M.},
	editor = {Neves, N. M. and Reis, R. L.}
}